Measuring retinal fluorescein leakage into the human vitreous with a modified confocal scanning laser ophthalmoscope.

Bernardes R., Isidoro J., Simões P., Lobo C., Cunha-Vaz J.

A method is described to quantify blood-retinal barrier permeability to fluorescein. Data is collected from the vitreous of the human eye at two elapsed time intervals after intravenous fluorescein administration, using a modified confocal scanning laser ophthalmoscope. Data processing includes fundus image enhancement, image alignment, profile construction, profile filtering and interpolation, exponential fit and integration. Automatic determination of the center of fovea is obtained by a correlation process. Grid overlapping on fundus images and permeability maps allows for matching chorioretinal morphological structures with alterations of blood -retinal barrier permeability to fluorescein.

Proceedings of the European Society of Engineering and Medicine. 1999, 5: 181-182.

1999